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N6876

Sigma-Aldrich

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

Synonym(s):
2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
Molecular Weight:
817.64
Beilstein:
4115923
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥90.0% (HPLC)

form

powder

impurities

≤10% solvent

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

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Application

  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
[59]
  • It has been used as a substrate for western blot detection.
  • [69]
  • It has been used for the staining of histological tissue sections.
  • [61]

    Packaging

    10×10, 50, 100, 250, 500 mg in glass insert
    1, 5 g in glass bottle

    Biochem/physiol Actions

    Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.[62][69]

    Substrates

    Substrate for dehydrogenases[48] and other oxidases.[49]

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    Pictograms

    Exclamation markHealth hazard

    Signal Word

    Warning

    Hazard Classifications

    Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

    Target Organs

    Respiratory system

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

    Enter Lot Number to search for Certificate of Origin (COO).

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