N6876

Sigma-Aldrich

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

Synonym(s):
3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, p-Nitrotetrazolium blue, Nitro BT, NBT, p-Nitro-Blue tetrazolium chloride
Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
Molecular Weight:
817.64
Beilstein/REAXYS Number:
4115923
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥90.0% (HPLC)

form

powder

application(s)

hematology: suitable
histology: suitable

impurities

≤10% solvent

solubility

H2O: 10 mg/mL

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

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Application

  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
  • It has been used as a substrate for western blot detection.
  • It has been used for the staining of histological tissue sections.

Packaging

10×10, 50, 100, 250, 500 mg in glass insert
1, 5 g in glass bottle

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Substrates

Substrate for dehydrogenases and other oxidases.

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Pictograms

Exclamation markHealth hazard

Signal Word

Warning

hazcat

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

Target Organs

Respiratory system

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus ?annamei.
Knaap V et al.
Aquaculture (Amsterdam, Netherlands), 191, 89-107 (2000)
Expression of the endothelial lipase gene in murine embryos and reproductive organs.
Lindegaard ML et al.
Journal of Lipid Research, 46, 439-439 (2005)
Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump.
Gaxiola RA et al.
Proceedings of the National Academy of Sciences of the USA, 98, 11444-11444 (2001)
Jens Madsen et al.
American journal of respiratory cell and molecular biology, 29(5), 591-597 (2003-06-05)
Lung surfactant protein A (SP-A) is a collectin produced by alveolar type II cells and Clara cells. It binds to carbohydrate structures on microorganisms, initiating effector mechanisms of innate immunity and modulating the inflammatory response in the lung. Reverse transcriptase-polymerase...
Detection of oxidases on polyacrylamide gels.
R N Feinstein et al.
Analytical biochemistry, 56(2), 353-360 (1973-12-01)
Articles
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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