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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

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Methanol, analytical standard
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(±)-Epichlorohydrin, purum, ≥99% (GC)
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Chloroform, analytical standard
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Acetone, analytical standard
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Formaldehyde solution, tested according to Ph. Eur.
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Tetrahydrofuran, analytical standard
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N,N-Dimethylformamide, analytical standard
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Sodium chloride solution, 5 M
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N,N-Dimethylformamide, for molecular biology, ≥99%
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N,N-Dimethylformamide, ACS reagent, ≥99.8%
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Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Methanol, anhydrous, 99.8%
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Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
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Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%
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Formaldehyde solution, for molecular biology, 36.5-38% in H2O
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Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
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N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
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2-Mercaptoethanol, BioUltra, for molecular biology, ≥99.0% (GC)
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1-Octanol, ≥98%, FCC, FG
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1-Octanol, anhydrous, ≥99%
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1-Octanol, natural, ≥98%, FCC
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1-Octanol, analytical standard
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Diethylene glycol, analytical standard
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