MilliporeSigma

Plasmid Purification & Isolation Kits

Bacterial plasmid DNA purification for molecular cloning, transcription, transformation, recombinant protein expression, and PCR applications

Plasmid purification is commonly used for molecular cloning, transformation, and recombinant protein expression. Plasmid purification kits are available based on size of the bacterial culture and corresponding plasmid yield (miniprep, midiprep, maxiprep, megaprep, and gigaprep).



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GenElute™ plasmid miniprep kits

GenElute™ plasmid miniprep kits offer simple, rapid, and cost-effective methods for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology and the convenience of a spin column format.

Features:

  • 40% more preps per kit than the leading supplier
  • Purify up to 20 mg of plasmid DNA per mL of culture
  • Purified plasmid DNA in less than 30 minutes for up to 24 preps
  • No phenol/chloroform extraction or alcohol precipitation required

GenElute™ HP plasmid prep kits

GenElute™ HP plasmid purification kits yield high quality plasmid DNA in less than 30 minutes for Mini, Midi, and Maxiprep kits, or 1.5 hours for Mega and Gigaprep kits.

  • From harvested bacterial culture to pure plasmid DNA in less time
  • Up to 25 mg (Mini), 350 mg (Midi), 1.2 mg (Maxi), 5 mg (Mega), and 15 mg (Giga) yield of high-copy plasmid DNA
  • Offers the flexibility of a vacuum or spin format (Mega and Gigaprep in vacuum format only)
  • No phenol/chloroform extraction or alcohol precipitation required
  • Kits are stable at room temperature for convenient storage

GenElute™ endotoxin-free plasmid prep kits

Endotoxins reduce cell viability and transfection efficiencies among different eukaryotic cell lines and can influence the outcome and reproducibility of a given transfection experiment when using mammalian and other eukaryotic cells. GenElute™ endotoxin-free plasmid prep kits offer a simple, rapid, cost-effective method for purifying plasmid DNA with <0.1

EU/mg DNA for high-efficiency transfection.

  • Higher transfection efficiency and yield than market leaders
  • Purify endotoxin-free plasmid DNA (≤0.1 EU/mg DNA)

GenElute™ five-minute plasmid miniprep kits

GenElute™ five-minute miniprep kits feature a streamlined protocol yielding up to 5 mg high-quality plasmid DNA in about five minutes.

  • Only 5 minutes from start to finish
  • No pelleting cells or clearing lysates
  • Binding column works with any standard laboratory vacuum manifold
  • Choose vacuum or spin protocols