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Plasmid Purification & Isolation Kits

Bacterial plasmid DNA purification for molecular cloning, transcription, transformation, recombinant protein expression, and PCR applications

Plasmid purification is commonly used for molecular cloning, transformation, and recombinant protein expression. Plasmid purification kits are available based on size of the bacterial culture and corresponding plasmid yield (miniprep, midiprep, maxiprep, megaprep, and gigaprep).

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Product Number
Product Description
Pricing
PLN350
GenElute™ Plasmid Miniprep Kit, sufficient for 350 purifications
PLN70
GenElute™ Plasmid Miniprep Kit, sufficient for 70 purifications
NA0200
GenElute™ HP Plasmid Midiprep Kit, sufficient for 25 purifications
NA0310
GenElute™ HP Plasmid Maxiprep Kit, sufficient for 25 purifications
03143414001
Genopure Plasmid Midi Kit, kit of for 20 isolations from 5 to 30 ml
HPPIKRO
High Pure Plasmid Isolation Kit, Roche, pkg of 50 purifications (11754777001), pkg of 250 purifications (11754785001)
NA0410
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit, sufficient for 25 preparations
PLED35
GenElute™ Endotoxin-free Plasmid Midiprep Kit, sufficient for 35 purifications
NA0160
GenElute™ HP Plasmid Miniprep Kit, sufficient for 350 preparations
03143422001
Genopure Plasmid Maxi Kit, kit of for 10 isolations from 30 to 150 ml
PLD35
GenElute™ Plasmid Midiprep Kit, sufficient for 35 purifications
NA0800
GenElute™ HP Select Plasmid Gigaprep Kit, sufficient for 5 preparations
NA0300
GenElute™ HP Plasmid Maxiprep Kit, sufficient for 10 purifications
PLEX15
GenElute™ Endotoxin-free Plasmid Maxiprep Kit, sufficient for 15 purifications
NA0100
PhasePrep™ BAC DNA Kit, Scalable method for isolating large-molecular weight plasmids
NA0150
GenElute™ HP Plasmid Miniprep Kit, sufficient for 70 preparations
LSKP096
Plasmid Miniprep96 Kit, Plasmid & BAC Miniprep
NA0400
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit, sufficient for 10 preparations
NA9604
GenElute™ HP 96-Well Plasmid Miniprep Kit, sufficient for 4 96-well plate purifications
PLX15
GenElute™ Plasmid Maxiprep Kit, sufficient for 15 purifications
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GenElute™ plasmid miniprep kits

GenElute™ plasmid miniprep kits offer simple, rapid, and cost-effective methods for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology and the convenience of a spin column format.

Features:

  • 40% more preps per kit than the leading supplier
  • Purify up to 20 mg of plasmid DNA per mL of culture
  • Purified plasmid DNA in less than 30 minutes for up to 24 preps
  • No phenol/chloroform extraction or alcohol precipitation required

GenElute™ HP plasmid prep kits

GenElute™ HP plasmid purification kits yield high quality plasmid DNA in less than 30 minutes for Mini, Midi, and Maxiprep kits, or 1.5 hours for Mega and Gigaprep kits.

  • From harvested bacterial culture to pure plasmid DNA in less time
  • Up to 25 mg (Mini), 350 mg (Midi), 1.2 mg (Maxi), 5 mg (Mega), and 15 mg (Giga) yield of high-copy plasmid DNA
  • Offers the flexibility of a vacuum or spin format (Mega and Gigaprep in vacuum format only)
  • No phenol/chloroform extraction or alcohol precipitation required
  • Kits are stable at room temperature for convenient storage

GenElute™ endotoxin-free plasmid prep kits

Endotoxins reduce cell viability and transfection efficiencies among different eukaryotic cell lines and can influence the outcome and reproducibility of a given transfection experiment when using mammalian and other eukaryotic cells. GenElute™ endotoxin-free plasmid prep kits offer a simple, rapid, cost-effective method for purifying plasmid DNA with <0.1

EU/mg DNA for high-efficiency transfection.

  • Higher transfection efficiency and yield than market leaders
  • Purify endotoxin-free plasmid DNA (≤0.1 EU/mg DNA)

GenElute™ five-minute plasmid miniprep kits

GenElute™ five-minute miniprep kits feature a streamlined protocol yielding up to 5 mg high-quality plasmid DNA in about five minutes.

  • Only 5 minutes from start to finish
  • No pelleting cells or clearing lysates
  • Binding column works with any standard laboratory vacuum manifold
  • Choose vacuum or spin protocols
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RNA Purification & Isolation Kits
Rapid, reliable total RNA and mRNA purification from a variety of samples including cells, tissues, viruses, plants, serum, and plasma.
Reaction Clean-Up
Purify nucleic acid from agarose gels, polyacrylamide gels, restriction digests, PCR reactions, and sequencing reactions with DNA and RNA cleanup purification kits for low to high throughput applications.
gDNA Purification & Isolation Kits
High yield, high quality DNA extraction and purification kits tailored to a variety of common and challenging sample types including cell culture, mammalian tissue, blood, viruses, bacteria, plant tissue, soil, water, urine, stool, PCR reactions, and agarose gel extractions.
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DNA and RNA gel electrophoresis reagents and Northern and Southern blotting tools for molecular biology and genomics research.
Molecular Cloning & Protein Expression
Molecular cloning and protein expression methods and protocols for reliable cloning and robust protein expression from insect, bacteria, and mammalian protein expression vectors.
Transfection
Transfection reagents, protocols, and resources including Roche X-tremeGENE transfection reagents for DNA, siRNA, miRNA, and CRISPR/Cas9 gene editing components.
PCR Reagents & Kits
Polymerase Chain Reaction (PCR) is a mainstay in molecular biology labs. PCR is used for amplifying DNA by several orders of magnitude and our reagents and resources are suitable for numerous research workflows including, gene expression analysis, genotyping, sequencing, and mutagenesis application needs.
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Discover a comprehensive portfolio of DNA oligo products including primers, qPCR probes, high-throughput DNA in plates and next-generation sequencing (NGS) adapters available in multiple formats and manufactured under ISO 9001 and ISO 13485 quality systems for your research or commercial applications.
Related Applications
DNA & RNA Purification
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.
Nucleic Acid Gel Electrophoresis
Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge for cloning, PCR, mass spectrometry, next generation sequencing (NGS), and Northern and Southern blotting applications.
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
qPCR
Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.
Sequencing
DNA sequencing reagents and tools for Sanger sequencing methods, DNA primers, probes, and additional nucleic acid sequencing applications.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
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