PCR stands for Polymerase Chain Reaction and is a mainstay of virtually every molecular biology lab. PCR is an easy and affordable method for amplifying specific fragments of DNA by several orders of magnitude. We have specialized kits for a variety of PCR, qPCR, and RT-PCR applications throughout your PCR workflow. Additionally, explore our comprehensive offering of PCR-related resources, including the Standard PCR Protocol and PCR Master Mix Calculator tool.
Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation. Browse our wide selection of Hot Start PCR enzymes including KOD, FastStart™, JumpStart™, and AptaTaq. For additional information on Hot Start PCR, product information and protocols, please visit our PCR Selection Guide and select the right product for your research needs.
In order to advance your molecular biology research, it is critical to maximize the efficiency of your workflow in a multitude of areas including, genomics, proteomics, and cellular analyses. As a pioneer in genomics and PCR technology, Roche’s complete suite of reagents and kits are optimized to reduce hands-on time at the bench, expedite data acquisition and the creation of key tools to test the most challenging of biological hypotheses. With a mutual drive for product quality and scientific excellence, our partnership allows us to bring Roche products to you, so the answers are there when you need them, wherever your work takes you. Explore all Roche PCR-related reagents and protocols on the Roche Products and Molecular Solutions resource page.
Quantitative PCR (qPCR) uses the linearity of DNA amplification to determine absolute or relative amounts of a known sequence in a sample. By using a fluorescent reporter in the reaction, it is possible to measure DNA generation in real time. In qPCR, DNA amplification is monitored at each cycle of PCR. When the DNA is in the log linear phase of amplification, the amount of fluorescence increases above the background. The point at which the fluorescence becomes measurable is called the Threshold cycle (CT) or crossing point. By using multiple dilutions of a known amount of standard DNA, a standard curve can be generated of log concentration against CT. The amount of DNA or cDNA in an unknown sample can then be calculated from its CT value.
Based on a highly optimized ReadyMix™ chemistry, LuminoCt™ delivers unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. LuminoCt™ works seamlessly with most qPCR instruments, so optimization of reaction conditions is kept to an absolute minimum. Often, users can simply choose their kit, build their assay, and begin. Generating the best possible qPCR data has never been faster or easier.
KiCqStart® ReadyMix™ reagents are ready-to-use, highly sensitive master mixes containing all the basic components for qPCR or RT-qPCR. Simply add your primers, probe and water to complete the assay cocktail. Add the cocktail to tubes or plates and then add your template. The KiCqStart® ReadyMix™ reagents are compatible with conventional or fast PCR mode. For more details, see product usage information at KiCqStart® Probe qPCR ReadyMix™ or KiCqStart® One-Step Probe RT-qPCR ReadyMix™ reagents. Additionally, the JumpStart Taq ReadyMixes provide a convenient solution for conventional qPCR experiments. The JumpStart Taq antibody delivers antibody-inactivated hot-start PCR which prevents non-specific product formation. Suitable for SYBR® Green and probe-based detection methods, the product line features options for optimization and offer compatibility with tube-, plate-, and capillary-based instruments. For additional information, please explore our additional SYBR® Green based qPCR reagents and resources.
RT-PCR combines two powerful and versatile techniques, reverse transcription and the polymerase chain reaction to generate and amplify cDNA from total RNA or mRNA transcripts. The method is often used to study gene expression at both the RNA and protein levels. The ideal RT-PCR requires a sensitive reverse transcription and a high fidelity amplification. The reverse transcriptase should be able to detect very low abundance transcripts and/or transcripts containing difficult secondary structure. Our enhanced Avian (eAMV™) Reverse Transcriptase displays all of these characteristics. It is the ideal RT for detecting low abundance messages that may be missed by other reverse transcriptases and it is the best enzyme we have found for transcribing through difficult secondary structure. It is also more tolerant to elevated temperatures than standard AMV, M-MLV, M-MLV RNase H– or AMV RNase H reduced. For additional product information and protocols, please explore our RT-PCR page or the comprehensive offering of PCR-related resources below.