RNA purification is a critical first step in many analyses due to the easy degradability of RNA. GenElute™ RNA purification kits enable purification of RNA from a variety of sources quickly and efficiently with minimal degradation, maximum purity, and high recovery and yield. GenElute™-E Single Spin RNA purification kits can be used to clean up RNA preps in a single spin for PCR and downstream applications.
Classic GenElute™ RNA purification kits use silica or silicon carbide to strongly bind RNA, allowing proteins and other biological contaminants to be removed through wash steps. mRNA purification kits use oligo dT30 covalently linked to polystyrene beads to capture polyadenylated mRNA by hybridization. After washing, purified RNA is eluted. A broad portfolio of GenElute™ purification kits tailored to specific sample types are available, enabling purification of total RNA and mRNA from cells, tissues, serum, plasma, bacteria, viruses, and plant tissue.
GenElute™-E RNA purification kits use a negative chromatography technique dependent on size exclusion to isolate and purify nucleic acid in a single spin. Smaller molecules such as proteins become trapped in the pores of the stationary phase of the column. Larger RNA molecules flow through the column quickly because they are too large to enter the pores. By exploiting this difference in size, GenElute™-E purification kits enable RNA purification in a single spin without the need for multiple binding and wash steps.
A variety of reagents are available to protect RNA from degradation. Stabilyser™ reagent is a proprietary formulation of detergents and solutes optimized for efficient extraction, stabilization, and storage of protein, DNA, and RNA from tissue samples. RNaseZAP™ reagent is a cleaning agent for removing RNase from glassware, plastic surfaces, countertops, and pipettors. RNAlater® reagent is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA through RNase inactivation. RNAstable® reagent is used to coat the bottom of storage tubes or wells, protecting picogram to microgram amounts of RNA at room temperature.