ZRB1500
Anti-Claudin 6 Antibody, clone 1B22 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Skullin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1B22, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 23.29 kDa
observed mol wt ~18 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CLDN6(9074)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 1B22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Claudin-6. It targets an epitope within 16 amino acids from the C-terminal, cytoplasmic domain.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human Claudin 6.
Application
Affinity Binding Assay: A representative lot of this antibody bound Claudin 6 peptide with a KD of 2.7 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Claudin 6 in human kidney and human stomach tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Claudin 6 in HUVEC and A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Claudin 6 in human kidney and human stomach tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Claudin 6 in HUVEC and A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Claudin 6, clone 1B22 ZooMAb, Cat. No. ZRB1500, is a recombinant Rabbit monoclonal antibody that detects Claudin 6 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Claudin-6 (UniProt: P56747; also known as Skullin) is encoded by the CLDN6 (also known as UNQ757/PRO1488) gene (Gene ID: 9074) in human. Claudin-6 is a multi-pass membrane protein that plays a major role in tight junction-specific obliteration of the intercellular space. Claudin-6 has two extracellular domains (aa 29-81 and 138-160), four transmembrane domains (aa 8-28, 82-102, 117-137, and 161-181), and three cytoplasmic domains (aa 1-7; 103-116, and 182-220). It acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells. Claudin-6 is expressed in the liver, in peripheral blood mononuclear cells, and hepatocarcinoma cell lines. It is also expressed specifically in undifferentiated stem cells and can serve as a marker to purify stem-like cells from a mixed cell population. It is shown to play an important role during mouse embryonic epithelium formation and the development of endodermal tissues and its level of expression is correlated with dedifferentiation and malignant proliferation of epithelial cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, L., et al. (2012). Cell Res. 22; 1082-1085; Zheng, A., et al. (2007). J. Virol. 81(22); 12465-12471).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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