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HPA011562

Sigma-Aldrich

Anti-TOMM20 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):
Anti-Mitochondrial import receptor subunit TOM20 homolog antibody produced in rabbit, Anti-Mitochondrial 20 kDa outer membrane protein antibody produced in rabbit, Anti-Outer mitochondrial membrane receptor Tom20 antibody produced in rabbit
Human Protein Atlas Number:

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μL

enhanced validation

orthogonal RNAseq
Learn more about Antibody Enhanced Validation

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:500-1:1000

immunogen sequence

KRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDV

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TOMM20(9804)

General description

Translocase of outer mitochondrial membrane 20 (TOMM20) is mapped to human chromosome 1q42.3. It comprises a N-terminal hydrophobic transmembrane (TM) segment and cytosol exposed C-terminal domain. TOMM20 also has glutamine rich acidic regions and a tetratrico peptide repeat (TPR).

Immunogen

Mitochondrial import receptor subunit TOM20 homolog recombinant protein epitope signature tag (PrEST)

Application

Anti-TOMM20 antibody produced in rabbit has been used in:
  • western blotting
  • confocal laser microscopy
  • immunofluorescence

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
Anti-TOMM20 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org). Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

TOMM20 (Translocase of outer mitochondrial membrane 20) is a major mitochondrial protein receptor involved in the cytosolic protein import phenomena. It is localized in the cytoplasm of spiral ganglia neurons. It functions as a subunit of a protein import complex to identify and translocate specific cytosolic proteins into the mitochondrial outer membrane. Study shows that TOMM20 acts as a biomarker in identifying the mitochondrial mass and oxidative phosphorylation in several cancer types.
TOMM20 anchors on the mitochondrial outer membrane via its N-terminal domain. The C-terminal region functions as a receptor for mitochondrial precursor proteins. The tetratrico peptide repeat (TPR) mediates protein-protein interactions. TOMM20 interacts with α-synuclein leading to the inhibition of mitochondrial protein import. Aberration in this interaction is implicated in the pathogenesis of Parkinson′s disease.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST72221.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Dynamin 1-like-dependent mitochondrial fission initiates overactive mitophagy in the hepatotoxicity of cadmium
Pi H, et al.
Autophagy, 9(11), 1780-1800 (2013)
Hodgkin Lymphoma: Metabolic Symbiosis Between Malignant Cells and Cancer-Associated Stromal Tissue
Blood, 122(21), 2993-2993 null
Kathryn Taggart et al.
BioResearch open access, 6(1), 182-191 (2017-12-30)
The Doberman pinscher (DP) canine breed displays a high incidence of idiopathic, nonischemic dilated cardiomyopathy (DCM) with increased mortality. A common mutation in DPs is a splice site deletion in the pyruvate dehydrogenase kinase 4 (PDK4) gene that shows a
Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20
Abe Y, et al.
Cell, 100(5), 551-560 (2000)
Zhaoying Yang et al.
Journal of cell science, 131(1) (2017-11-22)
Mitochondria-ER contact sites (MERCs) enable communication between the ER and mitochondria and serve as platforms for many cellular events, including autophagy. Nonetheless, the molecular organization of MERCs is not known, and there is no bona fide marker of these contact

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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