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ZRB1492

Sigma-Aldrich

Anti-RAD51 Antibody, clone 1M8 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
DNA repair protein RAD51 homolog 1, HsRAD51, RAD51 homolog A, hRAD51

biological source

rabbit (recombinant)

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1M8, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 36.97 kDa
observed mol wt 37 kDa

species reactivity

mouse, rat, human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... RAD51(5888)

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1M8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects RAD51 protein.

Immunogen

His-tagged full-length recombinant human RAD51 protein.

Application

Anti-RAD51, clone 1M8 ZooMAb, Cat. No. ZRB1492, is a recombinant Rabbit monoclonal antibody that specifically targets RAD51 and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RAD51 in HeLa and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RAD51 in human testis tissue sections.

Affinity Binding Assay Analysis: 100 μg/mL from a representative lot detected RAD51 in RAD51 and GST protein showed a KD of 1.0 x 10^-12 whereas binding of ZRB1492 to non-specific GST protein showed a KD of 1.7 x 10^-3.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RAD51 in HeLa, A431, HepG2, and NIH 3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Nuclear factor erythroid 2-related factor 2 (UniProt: Q16236; also known as NF-E2-related factor 2, NFE2-related factor 2, Nrf-2, HEBP1, Nuclear factor, erythroid derived 2, like 2) is encoded by the NFE2L2 (also known as NRF2) gene (Gene ID: 4780) in human. Nrf2 is a cap n collar basic-region leucine zipper transcription factor that that heterodimerizes with small Maf proteins. It is involved in adaptation of cells to oxidative stress and electrophiles. It binds to antioxidant response (ARE) elements in the promoter regions of target genes and induces a set of cytoprotective genes that are involved in the synthesis of reduced glutathione (GSH), repair of oxidized protein thiols, scavenging of reactive oxygen species, and drug metabolism. It is cytosolic under unstressed conditions, but translocates into the nucleus upon induction by electrophilic agents. Under normal unstressed conditions it is rapidly turned over in a Keap-1 dependent manner through Cul3-Rbx1 ubiquitination and proteasomal degradation. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates Nrf2 from its Keap-1 and promotes its translocation into the nucleus. It is widely expressed with highest expression in liver, skeletal muscle, luminal cells of the stomach and intestine, lining of the bronchi and alveoli, and in renal tubules; followed by heart, spleen, testis and brain. Nrf2 is constitutively upregulated in many tumors due to somatic mutations in the Keap1 or Nrf2 genes. Tumors over-expressing Nrf2 exhibit increased resistance to chemotherapeutic drugs and higher rates of proliferation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hayes, JD., and Ashford, MLJ (2012). Cell Metab. 16(2): 139-141).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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