M4276

Sigma-Aldrich

Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

clone MY-32, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

MY-32, monoclonal

contains

15 mM sodium azide

species reactivity

chicken, rabbit, feline, mouse, rat, bovine, human, guinea pig

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using skeletal muscle tissue
indirect immunofluorescence: 1:400
western blot: 1:1,000 using rabbit leg muscle extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

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General description

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.
Monoclonal Anti-Skeletal Myosin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin is a 480,000 dalton protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. These are designated at type I (slow twitch) and type II (fast-twitch). Type II fibers can be further subdivided in types IIA, IIB, and IIC.

Specificity

Monoclonal Anti-Skeletal Myosin is specific for the myosin heavy chain. It does not stain human or animal cardiac or smooth muscle myosin or cells grown by tissue culture (nonmuscle myosin). It has been demonstrated on human skeletal muscle that the antibody stains the fast twitch (type II) isomyosin molecules. Monoclonal Anti-Skeletal Myosin antibody does react with human rhabdomyosarcomas.

Immunogen

rabbit muscle myosin.

Application

The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.
Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunostaining
  • western blotting at a dilution 1:1000 and 1:90000†
  • indirect immunofluorescence (dilution 1:400) of formalin-fixed, paraffin-embedded sections of human or animal skeletal muscle tissue preparation.
  • dot immunobinding on muscle extracts or purified myosin preparations
Monoclonal Anti-Skeletal Myosin may be used for staining of human, rabbit, rat, mouse, bovine, chicken and guinea pig skeletal myosin. Monoclonal Anti-Skeletal Myosin antibody to fast-twitch skeletal myosin may be used for detecting cross striated muscle differentiation in tumors. The antibody localizes an epitope on the myosin chain that is stable to the routine formalin-fixation and paraffin-embedding process.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Espen E Spangenburg et al.
Journal of biomedicine & biotechnology, 2011, 598358-598358 (2011-10-01)
Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found...
Bleeding efficiency, microbiological quality and oxidative stability of meat from goats subjected to slaughter without stunning in comparison with different methods of pre-slaughter electrical stunning
Sabow AB, et al.
Testing, 11(4), e0152661-e0152661 (2016)
An optimized histochemical method to assess skeletal muscle glycogen and lipid stores reveals two metabolically distinct populations of type I muscle fibers
Prats C, et al.
PLoS ONE, 8(10), e77774-e77774 (2013)
Quantitative Human Physiology: An Introduction (2012)
S Cefalù et al.
Cell cycle (Georgetown, Tex.), 14(6), 894-901 (2015-03-20)
p53 family members, p63 and p73, play a role in controlling early stage of myogenic differentiation. We demonstrated that TAp63gamma, unlike the other p53 family members, is markedly up-regulated during myogenic differentiation in murine C2C7 cell line. We also found...

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