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05-636

Sigma-Aldrich

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

clone JBW301, Upstate®, from mouse

Synonym(s):
H2AXS139P, Histone H2A.X (phospho S139)
eCl@ss:
32160702

Quality Level

biological source

mouse

antibody form

affinity purified immunoglobulin

clone

JBW301, monoclonal

species reactivity

vertebrates

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

ambient

Related Categories

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Recognizes Histone H2A.X phosphorylated at Ser139.

Immunogen

peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X

Application

Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.

Quality

Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.

Target description

17 kDa

Linkage

Replaces: MABE205

Physical form

Protein G Purified
Format: Purified
Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.

Storage and Stability

Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Analysis Note

Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Quotes and Ordering

Assessment of chromosomal integrity using a novel live-cell imaging technique in mouse embryos produced by intracytoplasmic sperm injection.
Yamagata, K; Suetsugu, R; Wakayama, T
Human Reproduction null
DNA damage recognition in the rat zygote following chronic paternal cyclophosphamide exposure.
Barton, TS; Robaire, B; Hales, BF
Toxicological Sciences null
Lidia Ruiz et al.
PloS one, 3(9), e3230-e3230 (2008-09-19)
P53 activation can trigger various outcomes, among them reversible growth arrest or cellular senescence. It is a live debate whether these outcomes are influenced by quantitative or qualitative mechanisms. Furthermore, the relative contribution of p53 to Ras-induced senescence is also
Incomplete nuclear transformation of human spermatozoa in oligo-astheno-teratospermia: characterization by indirect immunofluorescence of chromatin and thiol status.
Ramos, L; van der Heijden, GW; Derijck, A; Berden, JH; Kremer, JA; van der Vlag, J; de Boer, P
Human Reproduction null
DNA interaction and dual topoisomerase I and II inhibition properties of the anti-tumor drug prodigiosin.
Montaner, B; Castillo-Avila, W; Martinell, M; Ollinger, R; Aymami, J; Giralt, E; Perez-Tomas, R
Toxicological Sciences null

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