XNACRL

Extract-N-Amp^™ Cellular RNA Lysis Buffer

Optimized for rapid cell lysis and RNA stabilization

• Synonym(s): RNA extraction buffer, RNA isolation buffer, RNA lysis buffer
• UNSPSC Code: 41106306

Properties

• Quality Level: 200
• form: liquid
• storage condition: OK to freeze (-10 to-25°C)
• suitability: suitable for PCR (suitable for RT-qPCR)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The Extract-N-AmpTM Cellular RNA Lysis buffer (XNACRL) is a single working solution that allows for rapid, room-temperature extraction and stabilization of RNA from a range of cells without heat, agitation or clean up. This is a simple and robust method for preparing cell lysate and stabilizing the extracted RNA for gene expression analysis directly from the cell lysate without the need for purification step prior to analysis.

Application

• The Extract-N-AmpTM Cellular RNA Lysis buffer is suitable for direct use with
• qRT-PCR
• RT-LAMP
• NGS library preparation

Features and Benefits

• From cell lysis to qRT-PCR in 15 minutes
• High Throughput and automation-friendly
• No RNA purification or clean up necessary
• No columns, heat, or centrifugation necessary
• Compatible with both adherent and non-adherent mammalian cell types
• Sensitivity range: 10-100k cells •May be paired with DNase I to ensure removal of DNA for more sensitive processes

Physical form

Liquid

Storage and Stability

Freezer (-10 to-25°C)

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• DNA-free JumpStart Taq DNA Polymerase 250U, 1.5KU
• DNA-free JumpStart Taq DNA Polymerase