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Tris Buffered Saline

10 ×, solution

MDL number:

Quality Level


0.2 μm filtered




10 ×

storage temp.


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Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.

Other Notes

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting. Working solution will contain 20 mM Tris, pH approx. 7.4, and 0.9% NaCl


Suitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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4-Chloro-1-Naphthol Solution Protocol for colorimetric detection of western blot proteins

Solution contains 0.48 mM 4-Chloro-1-Naphthol, 50 mM Tris-HCl and 0.2 M NaCl in 17% methanol. This is a substrate solution designed for visualizing horseradish peroxidase conjugates in Western Blotting.

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