All Photos(2)

T5030

Sigma-Aldrich

Tris Buffered Saline

tablet

Synonym(s):
TBS
MDL number:
NACRES:
NA.25

form

tablet

Quality Level

application(s)

diagnostic assay manufacturing

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Application

Tris Buffered Saline Tablets are suitable for use in immunoassay procedures.
It may be used in the following studies:
  • Immunogold-silver staining (IGSS) method to investigate the immunoglobulins in reactive human tonsil.
  • To study the aggregation of purified samples of α-synuclein.
  • As buffer in Western Blot analysis.
  • Time resolved fluoroimmunoassay.
  • As buffer in light microscopic immunohistochemistry.

Packaging

50, 100 tablets in poly bottle

Reconstitution

One tablet dissolved in 15 mL of deionized water yields 0.05 M Tris and 0.15 M sodium chloride, pH 7.6, at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

C S Holgate et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 31(7), 938-944 (1983-07-01)
A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed
HIV-1 infection leads to increased transcription of human endogenous retrovirus HERV-K (HML-2) proviruses in vivo but not to increased virion production.
Bhardwaj N, Maldarelli F, Mellors J, et al.
Journal of Virology, 88(19), 11108-11120 (2014)
G Pagès et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(18), 8319-8323 (1993-09-15)
The mitogen-activated protein kinases (MAP kinases) p42mapk and p44mapk are serine/threonine kinases rapidly activated in cells stimulated with various extracellular signals by dual phosphorylation of tyrosine and threonine residues. They are thought to play a pivotal role in integrating and
J E Meredith et al.
Molecular biology of the cell, 4(9), 953-961 (1993-09-01)
Programmed cell death (PCD) or apoptosis is a naturally occurring cell suicide pathway induced in a variety of cell types. In many cases, PCD is induced by the withdrawal of specific hormones or growth factors that function as survival factors.
Disease modifying effect of chronic oral treatment with a neurotrophic peptidergic compound in a triple transgenic mouse model of Alzheimer's disease.
Kazim SF, Blanchard J, Dai CL, et al.
Neurobiology of Disease, 71, 110-130 (2014)

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