MilliporeSigma
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T9039

Sigma-Aldrich

Tris Buffered Saline, with Tween® 20, pH 8.0

powder

Synonym(s):
TBST

form

powder

Quality Level

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Application

Tris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting.

Biochem/physiol Actions

Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique.

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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