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T9039

Sigma-Aldrich

Tris Buffered Saline, with Tween™ 20, pH 8.0

powder, pH 8.0, with Tween® 20

Synonym(s):

TBS, TBST

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

product name

Tris Buffered Saline, with Tween™ 20, pH 8.0, powder

form

powder

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Application

Tris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting.

Biochem/physiol Actions

Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique.

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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