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T2663

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

Synonym(s):

Tris hydrochloride solution

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About This Item

MDL number:
MFCD00012590
UNSPSC Code:
12161700
PubChem Substance ID:
24900064
NACRES:
NA.25

grade

for molecular biology
for molecular biology

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

7.35-7.45

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Fujii et al.
Journal of bacteriology, 154(2), 831-837 (1983-05-01)
The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell
B E Murray et al.
Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
M Kasahara et al.
The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)
El-Refaie Kenawy et al.
Journal of controlled release : official journal of the Controlled Release Society, 81(1-2), 57-64 (2002-05-07)
Electrospun fiber mats are explored as drug delivery vehicles using tetracycline hydrochloride as a model drug. The mats were made either from poly(lactic acid) (PLA), poly(ethylene-co-vinyl acetate) (PEVA), or from a 50:50 blend of the two. The fibers were electrospun
Diego Piacentini et al.
Environment international, 132, 105094-105094 (2019-09-05)
Over the last years, various acellular assays have been used for the evaluation of the oxidative potential (OP) of particular matter (PM) to predict PM capacity to generate reactive oxygen (ROS) and nitrogen (RNS) species in biological systems. However, relationships
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Protocols

10x Blocking Buffer Protocol

Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.

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