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SRP3329

Sigma-Aldrich

EGF from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):
URG, Urogastrone
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≥1x10^7 units/mg

mol wt

6.0 kDa

packaging

pkg of 500 μg

impurities

endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... EGF(13645)

General description

Epidermal growth factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[60] The gene encoding this protein is localized on mouse chromosome 3.[86] Recombinant murine EGF is a 6.0kDa globular protein containing 53 amino acid residues, including three intramolecular disulfide-bonds.

Biochem/physiol Actions

Epidermal growth factor (EGF) has been shown to inhibit gastric secretion, and to be involved in wound healing.[81][83] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[85] This receptor also binds with transforming growth factor-α (TGF-α) and VGF (vaccinia virus growth factor). Injection of EGF into neonatal and adult rats has been shown to induce neurobehavioral deficits related to schizophrenia.[58]

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 8.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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