SAB4200810
Anti-Podocin, Mouse monoclonal
clone PD-65, purified from hybridoma cell culture
Synonym(s):
NPHS2
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All Photos(3)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PD-65, monoclonal
form
buffered aqueous solution
mol wt
~42 kDa
species reactivity
bovine, rat, human, mouse
packaging
antibody small pack of 25 μL
concentration
1 mg/mL
technique(s)
immunoblotting: 0.25-0.5 μg/mL using using human HEK-293T cells.
immunofluorescence: 1-2 μg/mL using human HEK-293T cells.
isotype
IgG2a
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NPHS2(7827)
General description
Podocytes are highly specialized visceral epithelial cells that cover the outer aspect of the glomerulus in the kidney. The foot processes of adjacent podocytes regularly interdigitate leaving filtration slits that are bridged by a thin slit diaphragm. This is an extracellular adherens-like junction that serves as a barrier to macromolecules and plays a crucial role in the glomerular filtration process. Podocin (NPHS2 protein) is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band-7-stomatin family of lipid raft-associated proteins. It is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It interacts there with nephrin (NPHS1 protein), a protein critical in development and function of the kidney filtration barrier and the adapter protein CD2-AP. Podocin is anchored in the glycosphingolipids and cholesterol rich lipid rafts of the outer leaflet of the plasma membrane and is partially localized with the actin cytoskeleton. In vitro studies suggest its involvement in nephrin signaling facilitation via AP-1 in HEK cells. In addition to its presence in the kidney, it was also reported in rat nervous tissue and possibly in vascular smooth muscle.
Immunogen
Synthetic peptide of human Podocin , conjugated to KLH
Application
The antibody may be used in various immunochemical techniques including Immunobloting (~42kDa) and Immunofluorescence. Detection of the Podocin band by Immunoblotting is specifically inhibited by the immunogen.a
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Hiroshi Kawachi et al.
Journal of the American Society of Nephrology : JASN, 14(1), 46-56 (2002-12-31)
Podocin is identified as a product of the gene mutated in a patient with autosomal recessive steroid-resistant nephrotic syndrome. Although podocin is reported to be located at the slit diaphragm area, the precise role of podocin for maintaining the barrier
Séverine Roselli et al.
Traffic (Copenhagen, Denmark), 5(1), 37-44 (2003-12-17)
Podocytes are specialized epithelial cells of the glomerulus in the kidney, which interconnect at the top of the glomerular basement membrane through the slit diaphragm, an adherens-like junction that plays a crucial role in the glomerular filtration process. Podocin, a
Izumi Horinouchi et al.
Kidney international, 64(6), 2092-2099 (2003-11-25)
Mutations of the NPHS2 gene are responsible for autosomal-recessive steroid-resistant nephrotic syndrome. Its product, podocin, faces the slit diaphragm area with its two ends in the cytoplasm of foot processes. We generated rabbit polyclonal antibodies against conjugated peptides from human
G Caridi et al.
Journal of the American Society of Nephrology : JASN, 12(12), 2742-2746 (2001-12-01)
Podocin mutations (NPHS2 gene) are responsible for the autosomal recessive form of steroid-resistant nephrotic syndrome. As a result of a screening for these gene alterations in a cohort of Italian patients with nonfamilial nephrotic syndrome and histologic focal segmental glomerulosclerosis
Séverine Roselli et al.
Molecular and cellular biology, 24(2), 550-560 (2004-01-01)
Podocytes are specialized epithelial cells covering the basement membrane of the glomerulus in the kidney. The molecular mechanisms underlying the role of podocytes in glomerular filtration are still largely unknown. We generated podocin-deficient (Nphs2-/-) mice to investigate the function of
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