R8755
RPMI-1640 Medium
With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Roswell Park Memorial Institute 1640 medium
About This Item
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product name
RPMI-1640 Medium, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
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General description
Application
- Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
- Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
- Used for cell isolation and culture
- Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
- Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
- Used for the preparation of antifungal agents
Quantity
Reconstitution
Other Notes
also commonly purchased with this product
supplement
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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