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RPMI-1640 Medium

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Roswell Park Memorial Institute 1640 medium
MDL number:

Quality Level






cell culture | mammalian: suitable


endotoxin, tested


phenol red: yes
sodium pyruvate: no
L-glutamine: no
NaHCO3: yes

shipped in


storage temp.


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RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.


Supplement with 0.3 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

M Mathur et al.
JNMA; journal of the Nepal Medical Association, 53(198), 108-112 (2016-03-20)
Dermatophytosis is a common superficial fungal infection of the skin, hair and nails caused by Trichophyton, Microsporum and Epidermophyton dermatophytic species. Identification of causative dermatophytic species tend to vary with time and place and antifungal sensitivity is of epidemiological concern
Stepan Coufal et al.
Cells, 8(7) (2019-07-25)
Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them
Adela Ngwewondo et al.
PLoS neglected tropical diseases, 12(6), e0006565-e0006565 (2018-06-14)
Ivermectin is the only drug currently recommended for the treatment of onchocerciasis, the second leading infectious cause of blindness in the world. This drug kills only the first stage larvae-microfilariae (mf) of Onchocerca volvulus and is to be used cautiously
Grzegorz Sumara et al.
Cell, 136(2), 235-248 (2009-01-13)
Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of
Karen Verboom et al.
Nucleic acids research, 47(16), e93-e93 (2019-06-20)
Single cell RNA sequencing methods have been increasingly used to understand cellular heterogeneity. Nevertheless, most of these methods suffer from one or more limitations, such as focusing only on polyadenylated RNA, sequencing of only the 3' end of the transcript


Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine.


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