MilliporeSigma
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51536C

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RPMI-1640 Medium

with 2.05 mM L-glutamine, with 25mM HEPES, liquid, sterile-filtered, suitable for cell culture

MDL number:
NACRES:
NA.75

Quality Level

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

components

NaHCO3: 2000 mg/L
phenol red: 5.310 mg/L
L-glutamine: 300 mg/L
HEPES: 5958 mg/L

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used to incubate dissected lung tissue.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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