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ProteinA-Sepharose® 4, Fast Flow from Staphylococcus aureus

aqueous ethanol suspension

Protein A–Agarose
MDL number:

Quality Level

biological source

Staphylococcus aureus


aqueous ethanol suspension

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

extent of labeling

~6 mg per mL


affinity chromatography: suitable


Sepharose 4B Fast Flow

matrix activation

cyanogen bromide

matrix attachment


matrix spacer

1 atom


≥30 mg/mL binding capacity (human IgG)

storage temp.


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General description

Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.


Protein A-Sepharose® Fast Flow from Staphylococcus aureus has been used:
  • in co-immunoprecipitation assay
  • in immunodepletion
  • to purify IgG from human serum and plasma

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.


1 mL in glass insert
5 mL in glass bottle

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a registered trademark of Cytiva



Signal Word


Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids



Flash Point(F)

104.0 °F

Flash Point(C)

40 °C

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Yuhua Zhang et al.
Plant physiology, 149(4), 1860-1871 (2009-02-06)
Trehalose-6-phosphate (T6P) is a proposed signaling molecule in plants, yet how it signals was not clear. Here, we provide evidence that T6P functions as an inhibitor of SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11) of the SNF1-related group of protein kinases. T6P
H Sternlicht et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(20), 9422-9426 (1993-10-15)
A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled
Lydia Alvarez-Erviti et al.
Neurobiology of disease, 42(3), 360-367 (2011-02-10)
Alpha-synuclein aggregation plays a central role in Parkinson's disease pathology. Direct transmission of alpha-synuclein from pathologically affected to healthy unaffected neurons may be important in the anatomical spread of the disease through the nervous system. We have demonstrated that exosomes
Mazhar Adli et al.
Nature protocols, 6(10), 1656-1668 (2011-10-01)
Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions. However, conventional ChIP protocols necessitate the use of large numbers of cells, and library preparation steps associated with current high-throughput sequencing
Joyce A Benjamins et al.
Neurology(R) neuroimmunology & neuroinflammation, 6(3), e550-e550 (2019-05-03)
To identify whether factors toxic to oligodendrocytes (OLs), released by B cells from patients with MS, are found in extracellular microvesicles enriched in exosomes. Conditioned medium (Sup) was obtained from cultures of blood B cells of patients with MS and

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