P9424

ProteinA-Sepharose^® 4, Fast Flow from Staphylococcus aureus

aqueous ethanol suspension

• Synonym(s): Protein A–Agarose
• MDL number: MFCD00165563
• NACRES: NA.56

Properties

• biological source: Staphylococcus aureus
• Quality Level: 100
• form: aqueous ethanol suspension
• analyte chemical class(es): proteins (Immunoglobulins of various mammalian species)
• extent of labeling: ~6 mg per mL
• technique(s): affinity chromatography: suitable
• matrix: Sepharose 4B Fast Flow
• matrix activation: cyanogen bromide
• matrix attachment: amino
• matrix spacer: 1 atom
• capacity: ≥30 mg/mL binding capacity (human IgG)
• storage temp.: 2-8°C

Description

General description

Protein A-Sepharose^® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Application

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose^® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Protein A-Sepharose^® Fast Flow from Staphylococcus aureus has been used:

• in co-immunoprecipitation assay
• in immunodepletion
• to purify IgG from human serum and plasma

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(C): 40 °C