MilliporeSigma
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P3391

Millipore

Protein A-Sepharose® from Staphylococcus aureus

lyophilized powder

All Photos(1)
Synonym(s):
Protein A–Agarose
MDL number:
MFCD00165563
NACRES:
NA.56

form

lyophilized powder

Quality Level

200

packaging

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

storage condition

do not freeze

extent of labeling

3 mg per mL

technique(s)

affinity chromatography: suitable

color

white to gray

matrix

Sepharose CL-4B

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥16 mg/mL binding capacity (human IgG)

solubility

water: 1 g/L at 20 °C

suitability

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

storage temp.

2-8°C

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This Item
P6031P383882526
Millipore

Millipore

P3391

Protein A-Sepharose® from Staphylococcus aureus

Protein A from Staphylococcus aureus Soluble, essentially salt-free, lyophilized powder, extracellular

Sigma-Aldrich

P6031

Protein A from Staphylococcus aureus

Protein A from Staphylococcus aureus Soluble, Cowan Strain, essentially salt-free, lyophilized powder

Sigma-Aldrich

P3838

Protein A from Staphylococcus aureus

Sigma-Aldrich

Sigma-Aldrich

82526

Protein A soluble from Staphylococcus aureus (Cowan strain)

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

powder

packaging

glass bottle of 1 g, glass bottle of 1.5 g, glass bottle of 250 mg, glass bottle of 5 g

packaging

-

packaging

-

packaging

-

storage condition

do not freeze

storage condition

-

storage condition

-

storage condition

-

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

matrix

Sepharose CL-4B

matrix

-

matrix

-

matrix

-

solubility

water: 1 g/L at 20 °C

solubility

H2O: soluble 1 mg/mL, clear, colorless

solubility

-

solubility

H2O: 1 mg/mL, clear, colorless

General description

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Application

Protein A-Sepharose was used:
  • for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
  • in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
  • as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
  • in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose® is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Quantity

Swelling: 1 g swells to approx. 4 ml.

Physical form

Supplied as lyophilized powder stabilized with lactose and dextran.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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