Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.
Protein A-Sepharose was used:
- for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
- in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
- as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
- in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose® is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
250 mg in glass bottle
1, 1.5, 5 g in glass bottle
Swelling: 1 g swells to approx. 4 ml.
Supplied as lyophilized powder stabilized with lactose and dextran.
Sepharose is a registered trademark of Cytiva