P8615

Phosphoinositide 3-kinase p110γ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

• Synonym(s): PI3K p110γ human, Phosphatidylinositol 3-kinase p110γ human
• MDL number: MFCD03457993
• UNSPSC Code: 12352207
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected insect cells
• Quality Level: 200
• assay: ≥95% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ~4.5 units/mg protein
• UniProt accession no.: P48736
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PIK3CG(5294)

Description

General description

Phosphatidylinositol 3-kinase p110γ /phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit γ (PIK3CG) gene, with 12 exons spanning 43 kb on genomic DNA, is mapped to human chromosome 7q22.3. PIK3CG belongs to the family of genes coding for enzymes that phosphorylate the 3′-hydroxyl of phosphatidylinositol.

Biochem/physiol Actions

PI 3-kinases have been implicated in diverse cellular responses triggered by mammalian cell surface receptors. Inhibits NF-κB, AP-1, and STAT1α mediated gene expression induced by IFN-γ resulting in inhibition of COX-2 and NOS ll (iNOS) expression.

Phosphatidylinositol 3-kinase p110γ is implicated in the development of eosinophilic inflammation. Thus, inhibition of PI3Kγ expression can be considered as a potential therapeutic method for treating eosinophil-related diseases, including asthma. Knockdown of PIK3CG gene inhibits the PI3K-Akt/PKB signaling pathway, which is essential for tumorigenesis and the progression of colorectal cancers. Mutation in the gene is associated with the development of autistic disorder.

PI 3-kinases have been implicated in diverse cellular responses triggered by mammalian cell surface receptors. Inhibits NF-κB, AP-1, and STAT1α mediated gene expression induced by IFN-γ resulting in inhibition of COX-2 and NOS ll (iNOS) expression.

Unit Definition

1 unit will transfer 1 nmol of phosphate/min, using phosphatidylinositol as substrate.

Physical form

Solution in 10 mM HEPES, pH 7.5, 100 mM NaCl, 0.5 mM MgCl2, 50% glycerol.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable