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EZRMI

Millipore

Rat/Mouse Insulin ELISA Kit

measures and quantifies insulin levels in 10 μL serum or plasma

Synonym(s):

Insulin

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Rat/Mouse Insulin ELISA, This Rat/Mouse Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research.

Quality Level

200

species reactivity

mouse, rat

packaging

kit of 1 × 96 wells

parameter

10 μL sample volume (4hr assay)

assay range

accuracy: 83-102%
linearity: 79-133%
sensitivity: 0.2 ng/mL
standard curve range: 0.2-10 ng/mL

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma (K2 EDTA)

UniProt accession no.

P01322
P01325

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

General description

Insulin is secreted by the islet β cells in the pancreas. It contains two peptide chains linked by disulfide bridges. The A chain is composed of 21 amino acids and the B chain has 30 amino acids.

Application

Rat/Mouse Insulin ELISA is suitable for measuring and quantifying insulin levels in metabolism and endocrine research. It has been used to determine plasma insulin by enzyme-linked immunosorbent assay. It has also been used to measure the insulin secretion from cells, in vitro.

Biochem/physiol Actions

Insulin is a key hormone that maintains normal blood glucose levels. It mediates cellular glucose uptake, controls carbohydrate, lipid, and protein metabolism, and enhances cell division and growth through its mitogenic effects. Insulin resistance is a condition of impaired sensitivity to insulin-mediated glucose disposal.

Other Notes

3 hour assay, room temperature 10 μL sample size
Research Category: Metabolism
Research Sub Category: Obesity/Metabolic Disorders

Disclaimer

For research use only. Not for use in diagnostic procedures.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Warning

hcodes

H290,H317

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Offspring metabolomic response to maternal protein restriction in a rat model of intrauterine growth restriction (IUGR).
Alexandre-Gouabau, Marie-Cecile, et al.
Journal of Proteome Research, 10, 3292-3302 (2011)
Hypoxia-induced intrauterine growth restriction increases the susceptibility of rats to high-fat diet-induced metabolic syndrome.
Rueda-Clausen CF, Dolinsky VW, Morton JS, Proctor SD, Dyck JR, Davidge ST.
Diabetes null
Amadori-glycated phosphatidylethanolamine, a potential marker for hyperglycemia, in streptozotocin-induced diabetic rats.
Sookwong P, Nakagawa K, Fujita I, Shoji N, Miyazawa T.
Lipids null
How moderate changes in Akt T-loop phosphorylation impact on tumorigenesis and insulin resistance.
Wullschleger S, Sakamoto K, Johnstone L, Duce S, Fleming S, Alessi DR.
Disease models & mechanisms null
Chronic hypoxia increases insulin-stimulated glucose uptake in mouse soleus muscle.
Gamboa JL, Garcia-Cazarin ML, Andrade FH
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null
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