MilliporeSigma
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P6486

Millipore

EZview Red Protein A Affinity Gel

MDL number:
NACRES:
NA.56

form

liquid

Quality Level

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

capacity

~4 mg binding capacity (rabbit IgG)

shipped in

wet ice

storage temp.

−20°C

General description

EZview Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.

Application

EZview Red Protein A Affinity Gel has been used for purification of proteins in the preparation of immunoprecipitation and chromatin immunoprecipitation (ChIP) assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Packaging

1 mL in poly bottle

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Physical form

50% slurry in PBS with 50%Glycerol and 0.0015%
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Storage and Stability

EZview Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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