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M5546

Sigma-Aldrich

Monoclonal Anti-c-Myc antibody produced in mouse

clone 9E10, ascites fluid

Synonym(s):
Anti-c-Myc
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

9E10, monoclonal

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:100 using c-myc tagged fusion protein

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MYC(4609)

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General description

MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. c-myc is a member of MYC gene family. It is located on human chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
c-Myc is a transcription factor which is over-expressed in a wide range of cancers.

Specificity

Recognizes both N- terminal and C-terminal c-Myc tagged proteins.

Immunogen

synthetic peptide of the human p62c-Myc protein.

Application

Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry.
Monoclonal Anti-c-Myc antibody has also been used in:
  • the detection of horseradish peroxidase conjugate in phosphate buffered saline tween-20 (PBST) and 65-kDa mannoprotein (MP65) Dabs
  • immunoblotting
  • immunoprecipitation
  • biochemical and histological studies

Biochem/physiol Actions

c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc.
The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as a transcriptional regulator of gene expression. MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in the poor outcome and invasive treatment, when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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