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L4005

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

purified by trichloroacetic acid extraction

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Synonym(s):
LPS
EC Number:
297-473-0
MDL number:
MFCD00164401
NACRES:
NA.25

biological source

Escherichia coli (O55:B5)

Quality Level

200

form

lyophilized powder

purified by

trichloroacetic acid extraction

impurities

1-10% Protein (Lowry)

color

white to yellow

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

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This Item
L4130L4524L2630
Lipopolysaccharides from Escherichia coli O55:B5 purified by trichloroacetic acid extraction

Sigma-Aldrich

L4005

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O111:B4 purified by trichloroacetic acid extraction

Sigma-Aldrich

L4130

Lipopolysaccharides from Escherichia coli O111:B4

Lipopolysaccharides from Escherichia coli O55:B5 purified by ion-exchange chromatography, TLR ligand tested

Sigma-Aldrich

L4524

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O111:B4 purified by phenol extraction

Sigma-Aldrich

L2630

Lipopolysaccharides from Escherichia coli O111:B4

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

impurities

1-10% Protein (Lowry)

impurities

1-10% Protein (Lowry)

impurities

<1% Protein, <1% RNA

impurities

≤3.00% protein (Lowry-TCA)

color

white to yellow

color

white to light yellow

color

white to yellow cast

color

off-white to faint yellow

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

This product is TCA extracted from E. coli serotype O55:B5. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

related product

Product No.
Description
Pricing

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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O Schulz et al.
Mucosal immunology, 7(4), 892-904 (2013-12-19)
Lymphoid organ hypertrophy is a hallmark of localized infection. During the inflammatory response, massive changes in lymphocyte recirculation and turnover boost lymphoid organ cellularity. Intriguingly, the exact nature of these changes remains undefined to date. Here, we report that hypertrophy
A Fernandez-Bustamante et al.
Respiratory physiology & neurobiology, 183(2), 149-158 (2012-06-26)
The regional distribution of inflammation during acute lung injury (ALI) is not well known. In an ovine ALI model we studied regional alveolar inflammation, surfactant composition, and CT-derived regional specific volume change (sVol) and specific compliance (sC). 18 ventilated adult
J T Frieling et al.
Antimicrobial agents and chemotherapy, 41(7), 1439-1443 (1997-07-01)
The in vitro production of interleukin-1beta (IL-1beta), IL-6, and the IL-1 receptor antagonist (IL-1ra) in whole blood upon stimulation with different bacterial strains was measured to study the possible relationship between disease severity and the cytokine-inducing capacities of these strains.
Xiao-Wen Lin et al.
PloS one, 8(6), e67633-e67633 (2013-06-27)
Toll-like receptors (TLRs) play a pivotal role in the defense against invading pathogens by detecting pathogen-associated molecular patterns (PAMPs). TLR4 recognizes lipopolysaccharides (LPS) in the cell walls of Gram-negative bacteria, resulting in the induction and secretion of proinflammatory cytokines such
Chen Hu et al.
Cell biology international, 43(10), 1174-1183 (2019-01-12)
Regeneration of pulmonary epithelial cells plays an important role in the recovery of acute lung injury (ALI), which is defined by pulmonary epithelial cell death. However, the mechanism of the regenerative capacity of alveolar epithelial cells is unknown. Using a
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