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I9018

Sigma-Aldrich

Monoclonal Anti-Involucrin antibody produced in mouse

clone SY5, ascites fluid

Synonym(s):
Anti-IVL
MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SY5, monoclonal

contains

15 mM sodium azide

species reactivity

human, canine, gorilla, owl monkey, pig

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... IVL(3713)

General description

Involucrin is a soluble precursor protein that regulates terminal differentiation in keratinocytes. This protein is initially present in the cytosol component of keratinocytes and later associates with membrane proteins. Involucrin is expressed in squamous cell cancer and its expression is down-regulated by HPV 16 oncoproteins . Monoclonal Anti-Involucrin is specific for involucrin in primate and non-primate mammals. In cultured human keratinocytes, the antibody stains a 120 kDa band by immunoblot under denaturing-reducing conditions. However, in other cells the antibody binds to involucrin protein in varied sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approx. 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet at approx 105 kDa in pig. The antibody does not bind to mouse epidermis; hence the antibody can be used to study human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies .

Specificity

The antibody detects involucrin in a range of primate and nonprimate mammals in a variety of techniques. In immunoblotting of cultured human keratinocytes, the antibody stains a 120 kDa band under denaturing and reducing conditions. However, in other systems, the antibody stains the involucrin protein in a variety of sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approximately 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of ~105 kDa in pig. The antibody does not react with mouse epidermis, permitting use in studies of human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies.

Immunogen

human involucrin.

Application

Monoclonal Anti-Involucrin antibody is suitable for use in immunofluorescence at 1:100 dilutions using keratinocytes or for indirect immunofluorescence using methanol fixed mouse embryonic fibroblasts-keratinocytes co-cultures . The antibody may also be used for western blot (1:1000) and immunohistochemical analysis . Additionally, the product can be useful for indirect ELISA, immunoblotting, immunoprecipitation and immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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