MilliporeSigma
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I3146

Sigma-Aldrich

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):
Human ITS Liquid Media, Insulin-transferrin-sodium selenite
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Components

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Leonardo Ricotti et al.
Biomedical materials (Bristol, England), 6(3), 031001-031001 (2011-05-14)
Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro
Kelvin S C Cheung et al.
PloS one, 9(6), e98063-e98063 (2014-06-04)
MicroRNAs (miRs) play a pivotal role in a variety of biological processes including stem cell differentiation and function. Human foetal femur derived skeletal stem cells (SSCs) display enhanced proliferation and multipotential capacity indicating excellent potential as candidates for tissue engineering
Christine M Reich et al.
Veterinary research communications, 36(2), 139-148 (2012-03-07)
In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrow-derived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent
Cyrill Géraud et al.
Hepatology (Baltimore, Md.), 52(1), 313-326 (2010-06-26)
Liver sinusoidal endothelium (LSEC) is a prime example of organ-specific microvascular differentiation and functions. Disease-associated capillarization of LSEC in vivo and dedifferentiation of LSEC in vitro indicate the importance of the hepatic microenvironment. To identify the LSEC-specific molecular differentiation program
Loraine L Y Chiu et al.
The Laryngoscope, 127(3), E91-E99 (2016-11-20)
Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit

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