M4530

Medium 199

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): M199 Medium, TCM 199
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: yes; phenol red: yes; HEPES: no; sodium pyruvate: no; NaHCO₃: yes; Earle’s salts (5% CO₂): yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Complete liquid version of the original M 199 formulation published by Morgan.

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable