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H6648

Sigma-Aldrich

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
HBSS
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 1.0 g/L (Dextro)
phenol red: no
NaHCO3: 0.35 g/L

shipped in

ambient

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General description

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
Formulation: click here.

Application

Calcium and magnesium support cell adhesion. Used to minimize cell attachment or aggregation.
Hanks′ Balanced Salt solution has been used:

  • to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.
  • to measure the stability of the magnetic scaffold material in bone tissue engineering.
  • in Fluorometric Measurements of intracellular calcium levels [Ca2+]
  • for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood
  • For assessing airway inflammation in mice

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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