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H2387

SAFC

Hanks′ Balanced Salts

Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

HBSS

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About This Item

MDL number:
MFCD00217418
UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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General description

Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.

Application

Hanks′ Balanced Salt has been used:

  • to culture primary hippocampal neuron cells from rats
  • to collect the rat brains
  • to culture rat primary hepatocytes

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Yu-Kyoung Kim et al.
Scientific reports, 8(1), 13264-13264 (2018-09-07)
A polymer coating as polycaprolactone (PCL) is applied to improve the initial corrosion resistance of biodegradable magnesium. In addition, plasma electrolytic oxidation (PEO) is performed to increase adhesion between the polymer and the metal. However, when a complex-shaped material such
Shuoguo Li et al.
Journal of structural biology, 201(1), 63-75 (2017-11-09)
Cryo-correlative light and electron microscopy (cryo-CLEM) offers a unique way to analyze the high-resolution structural information of cryo-vitrified specimen by cryo-electron microscopy (cryo-EM) with the guide of the search for unique events by cryo-fluorescence microscopy (cryo-FM). To achieve cryo-FM, a
Seo Yeon Lee et al.
Polymers, 12(2) (2020-02-20)
Herein, we describe precisely a covalent modification of pure magnesium (Mg) surface and its application to induce in vitro osteogenic differentiation. The new concept of a chemical bonding method is proposed for developing stable chemical bonds on the Mg surface
Abdelrahman I Rezk et al.
Scientific reports, 9(1), 117-117 (2019-01-16)
Magnesium (Mg) and its alloys have attracted much attention as a promising candidate for degradable implant applications however the rapid corrosion of magnesium inside the human body greatly limits its use as an implant material. Therefore, coating the alloy surface
Yu Bai et al.
Journal of materials science. Materials in medicine, 27(2), 31-31 (2015-12-26)
Au nanoparticles (AuNPs) are successfully assembled on TiO2 nanotube (TN) arrays through electrochemical deposition technology to improve the surface characteristics of TN arrays as an implant material. The loading amount of AuNPs can be controlled by adjusting the deposition time
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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