G8529
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)
Synonym(s):
G-6-P-DH
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About This Item
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recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
≥550 units/mg protein (biuret)
composition
Protein, 10-40% biuret
application(s)
agriculture
foreign activity
creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
storage temp.
2-8°C
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Application
Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:
- in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
- to study the starch time course using Nicotiana tabacum leaves
- to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
- to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.
Unit Definition
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
Physical form
Lyophilized powder containing Ficoll and Tris buffer salts
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
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