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EXTRA2

Sigma-Aldrich

Mouse ExtrAvidin® Peroxidase Staining Kit antibody produced in goat

Synonym(s):

ExtrAvidin® Peroxidase Staining Kits

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

antibody product type

secondary antibodies

Quality Level

200

usage

sufficient for 2,000 tests ELISA, Immunoblotting
 sufficient for 200 tests Immunohistology

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

These kits comprise universal reagents for use with primary antibodies in immunohistology, ELISA, and immunoblotting.
ExtrAvidin® is a unique form of avidin, available only from Sigma, that combines the high specificity and affinity of avidin for biotin with low non-specific binding at physiological pH. ExtrAvidin® peroxidase exhibits high sensitivity with low background.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Features and Benefits

  • Use in immunohistology, ELISA, and immunoblotting assays.
  • Affinity Isolated Antibodies have been adsorbed with human IgG and IgM to minimize cross-reactivity.
  • Biotinylated antibodies contain a spacer which improves accessibility for the ExtrAvidin® conjugates.

Components

• 3 mL ExtrAvidin®-Peroxidase
• 3 mL Biotinylated Secondary Antibodies
• Complete Instructions and Assay Protocols

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sahil Adriouch et al.
Frontiers in microbiology, 2, 199-199 (2011-11-03)
Initially thought as being non-immunogenic, recombinant AAVs have emerged as efficient vector candidates for treating monogenic diseases. It is now clear however that they induce potent immune responses against transgene products which can lead to destruction of transduced cells. Therefore
Matheus L Medeiros et al.
Journal of inflammation research, 14, 6477-6489 (2021-12-10)
Methylglyoxal (MGO) is a highly reactive dicarbonyl species implicated in diabetic-associated diseases. Acute lung injury (ALI) symptoms and prognosis are worsened by diabetes and obesity. Here, we hypothesized that elevated MGO levels aggravate ALI, which can be prevented by metformin.
Tiago Savignon et al.
PloS one, 7(4), e35786-e35786 (2012-04-28)
Astrogliosis, oligodendroglial death and motor deficits have been observed in the offspring of female rats that had their uterine arteries clamped at the 18(th) gestational day. Since nitric oxide has important roles in several inflammatory and developmental events, here we
Matheus L Medeiros et al.
Journal of inflammation research, 14, 6477-6489 (2021-12-10)
Methylglyoxal (MGO) is a highly reactive dicarbonyl species implicated in diabetic-associated diseases. Acute lung injury (ALI) symptoms and prognosis are worsened by diabetes and obesity. Here, we hypothesized that elevated MGO levels aggravate ALI, which can be prevented by metformin.
Karīna Narbute et al.
Stem cells translational medicine, 8(5), 490-499 (2019-02-02)
Parkinson's disease (PD) is the second most common neurodegenerative disorder affecting millions of people worldwide. At present, there is no effective cure for PD; treatments are symptomatic and do not halt progression of neurodegeneration. Extracellular vesicles (EVs) can cross the
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