Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.
D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
- to dissolve Angelica gigas powder for microencapsulation
- to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
- to wash amniotic membrane for cellular analyses
Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.
Formulated to contain 9.6 grams of powder per liter of medium.
This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available