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D1408

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

10×, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
DPBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
glucose: no (Dextro)

shipped in

ambient

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General description

Dulbecco′s Phosphate Buffered Saline (DPBS) is a balanced salt solution.

Application

Dulbecco′s Phosphate Buffered Saline has been used:
  • in microfluidic cell culture of mouse embryonic stem cells (mESC)
  • to dissolve albumin for adsorption studies
  • in PC12 cell culture experiments

Dulbecco′s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water (W3500). D-PBS is used to wash cells during preparation and serial transfer.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Alexandre Super et al.
Biotechnology journal, 11(9), 1179-1189 (2016-05-24)
Oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. Quantification of cellular oxygen kinetics, i.e. the determination of specific oxygen uptake rates (sOURs), is routinely used to understand
Marjan Nabili et al.
Ultrasound in medicine & biology, 39(4), 638-646 (2013-02-19)
Delivery of sufficient amounts of therapeutic drugs into the eye is often a challenging task. In this study, ultrasound application (frequencies of 400 KHz to 1 MHz, intensities of 0.3-1.0 W/cm(2) and exposure duration of 5 min) was investigated to overcome
Frank Klont et al.
Talanta, 182, 414-421 (2018-03-05)
The study of proteins is central to unraveling (patho)physiological processes and has contributed greatly to our understanding of biological systems. Corresponding studies often employ procedures to enrich proteins from their biological matrix using antibodies or other affinity binders coupled to
Jean E Copper et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 208, 38-46 (2017-11-22)
In recognition of the importance of zebrafish as a model organism for studying human disease, we have created zebrafish content for a web-based reference atlas of microanatomy for comparing histology and histopathology between model systems and with humans (http://bio-atlas.psu.edu). Fixation
B Sterner et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 101, 126-136 (2016-02-16)
The treatment of joint related diseases often involves direct intra-articular injections. For rational development of novel delivery systems with extended residence time in the joint, detailed understanding of transport and retention phenomena within the joint is mandatory. This work presents

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