MilliporeSigma
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C6-BU-1

08062563

biological source

rat brain

growth mode

Adherent

karyotype

modal No. of chromosomes is 42

morphology

fibroblastic/fusiform

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat glioma x rat glioma hybrid

Cell Line Description

A 8-bromodeoxybromidine resistant mutant of the rat glioma cell line C6. Found to be tumourigenic in BALB/c nude mice and Wistar rats. Strain rattus norvegicus

Application

Used in the generation of cell hybrids.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C

Other Notes

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Certificate of Analysis

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Certificate of Origin

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