MilliporeSigma
All Photos(1)

C17.2

07062902

biological source

mouse brain

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse multipotent neural progenitor or stem-like cells

Cell Line Description

An immortalised mouse neural progenitor cell line capable of differentiation in vitro. The cell line was established by retorviral-mediated transduction of the avian myc oncogene into mitotic progenitor cells of neonatal mouse cerrebellum. Mouse strain CD1 x C57BL/6. This cell line is a valuable tool for in vitro and in vivo studies aimed at understanding the control of cell fate and differentiation of neural progenitors. The MMLV retrovirus vector used for the immortalisation process contained a neo resistance gene transcribed from an internal SV40 promoter. Therefore the cells are neo resistant. The morphology of the cells may change over time. Cells plated at low density may tend to become more process bearing whereas those plated more densely may tend to become flat and non-process bearing.

Culture Medium

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). Flasks should be pre-coated with poly-L-lysine at 10 μg/ml in sterile distilled water.

Subculture Routine

Feed cells weekly with 50% conditioned medium and 50% fresh medium or split 1:10-1:20 in fresh medium using trypsin/EDTA. 5% CO2; 37 °C. Split sub-confluent cultures 1:10 - 1:20, i.e., seeding at 2-4 x 10,000 cells/cm2. Flasks should be pre-coated with poly-L-lysine at 10 μg/ml in sterile distilled water. Cells can be split as dilute as 1:50, but the phenotype may change, i.e., cells may appear flat with an epithelial-like morphology which usually do not stain for neurofilament or GFAP.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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