Glutamate is a major excitatory neurotransmitter used as a signaling molecule between nerve cells and is involved in most aspects of normal brain function including cognition, learning and memory. Various glutamate receptors are found throughout the brain and include NMDA, AMPA/kainate and metabotropic receptors (mGluR). Normal glutamate levels are essential for normal brain function, however high levels may result in over-excitation of nerve cells, leading to eventual cell damage and/or cell death. Glutamate-induced cytotoxicity has been implicated in neurodegenerative disorders such as Alzheimer’s disease, Huntington’s disease and Parkinson’s disease along with other conditions including spinal cord injury and multiple sclerosis. Therapies that may inhibit or reduce the effects of glutamate activity are thus of great therapeutic interest.
HT-22 is an immortalized mouse hippocampal cell line subcloned from the HT-4 cell line . The parental HT-4 cell line was derived from the immortalization of mouse neuronal tissues with a temperature sensitive SV40 T-antigen . HT-22 is highly sensitive to glutamate and is thus frequently used as a model system to study glutamate-induced toxicity in neuronal cells.
Cell Line Description
Neural Lineage Cells
EMD Millipore has a limited license to sell this product for research use only, and not for any commercial uses. Excluded commercial uses include without limitation manufacturing, providing a service, therapeutic, diagnostic and prophylactic uses, and any other commercial uses. Use of this product by a purchaser for any purpose other than for research is unauthorized and prohibited.
Inquiries about licensing for commercial or other uses should be directed to:
Office of Technology Development
The Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla, CA 92037
Phone: (858) 453-4100 extension 1278
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.