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from rat brain, 09061601

biological source

rat brain

growth mode



Not specified




GPNT cells express tight junction proteins


P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I


cell culture | mammalian: suitable

shipped in

dry ice

Cell Line Origin

An immortalised Lewis rat brain vascular endothelial cell line

Cell Line Description

GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

DNA Profile

Not specified

Culture Medium

Hams F10 + 10% Heat-inactivated FCS + 2 mM Glutamine + 2 ng/ml basic fibroblast growth factor + 80 μg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10μg/cm2 from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to a mMonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 10hrs.

Other Notes

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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

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Certificate of Origin

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