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V5265

Sigma-Aldrich

Crystal violet solution

1%, aqueous solution

Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
Molecular Weight:
407.98
Beilstein:
3580948
MDL number:
PubChem Substance ID:

Quality Level

form

aqueous solution

concentration

1%

application(s)

hematology: suitable
histology: suitable

Amax

0.36-0.44 at 589-594 nm

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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General description

Crystal violet, also known as gentian violet, is a protein dye and is basic in nature. It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.

Application

Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Studies on adsorption of crystal violet dye from aqueous solution onto coniferous pinus bark powder (CPBP)
Ahmad R.
Journal of Hazardous Materials, 171, 767-773 (2009)
Characterization of potent and selective iodonium-class inhibitors of NADPH oxidases
Lu J, et al.
Biochemical Pharmacology, 43, 25-38 (2017)
Li Qi et al.
International journal of molecular medicine, 38(6), 1734-1742 (2016-10-18)
Prostate carcinoma is a devastating disease which is characterized by insidious early symptoms, rapid progression and a poor prognosis. Tripartite motif-containing protein 16 (TRIM16) was identified as an estrogen- and antiestrogen-regulated gene in epithelial cells stably expressing estrogen receptors. The protein...
K Engbaek et al.
Journal of clinical pathology, 32(2), 187-190 (1979-02-01)
Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be...
Viviana Bustos et al.
Oncotarget, 8(48), 84258-84275 (2017-11-16)
The estrogen receptor ERβ is the predominant ER subtype expressed in normal well-differentiated colonic epithelium. However, ERβ expression is lost under the hypoxic microenvironment as colorectal cancer (CRC) malignancy progresses. This raises questions about the role of signalling through other...

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