G2039

Gentian Violet

meets USP testing specifications

• Synonym(s): Crystal Violet, Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
• Linear Formula: C₂₅H₃₀N₃Cl
• CAS Number: 548-62-9
• Molecular Weight: 407.98
• Colour Index Number: 42555
• Beilstein/REAXYS Number: 3580948
• EC Number: 208-953-6
• MDL number: MFCD00011750
• UNSPSC Code: 12352100
• PubChem Substance ID: 24895083
• NACRES: NA.21

Properties

• agency: USP/NF; meets USP testing specifications
• Quality Level: 200
• mp: 205 °C (dec.) (lit.)
• antibiotic activity spectrum: fungi
• application(s): pharmaceutical (small molecule)
• mode of action: cell membrane | interferes; enzyme | inhibits
• SMILES string: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChI key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

General description

Gentian Violet, also known as crystal violet, is a synthetic triarylmethane dye. It is widely used in various applications, including histology, microscopy, and microbiology. It is often used to stain tissues, cells, and microorganisms for visualization and identification purposes.

Application

Gentian Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. Used as a component of CV cytotoxicity assays.

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H318,H351,H410
• pcodes: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves