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Key Documents

C6867

Sigma-Aldrich

Anti-CENP-A antibody produced in rabbit

enhanced validation

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CenH3, Anti-Centromere Protein A

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 17 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~2 mg/mL

technique(s)

western blot: 1-2 μg/mL using HEK-293 cells expressing human CENP-A

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CENPA(1058)

General description

CENP-A is a 17 kDa histone H3- like protein.
Centromere protein A is a protein encoded by the CENPA gene in humans. It is referred as CenH3 and CENP-A. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on a chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin.

Immunogen

synthetic peptide corresponding to amino acids 32-49 located near the N-terminus of human CENP-A, conjugated to KLH.

Application

Anti-CENP-A antibody has been used in immunoblotting.
Anti-CENP-A antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using HEK-293 cells expressing human CENP-A.

Biochem/physiol Actions

The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of (CENP-A) plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein ICP0
Lomonte P, et al.
Test, 276(8), 5829-5835 (2001)
Jun-Jun Qiu et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 34(5), 2971-2975 (2013-05-29)
Altered expression of centromere protein-A (CENP-A) is observed in various types of human cancers. However, the clinical significance and pathological role of CENP-A in epithelial ovarian cancer (EOC) remains unclear. The main objective of this investigation was to clarify the
R-M Liu et al.
Folia biologica, 59(3), 105-109 (2013-07-31)
Serine 7 of centromere protein A (CENP-A) is a very important mitosis-specific phosphorylation site. In this study, we demonstrate the subcellular distribution of Ser7 phosphorylated CENP-A during mitosis in MCF-7 cells. The Ser7 phosphorylation of CENP-A was observed beginning at
Aaron O Bailey et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(29), 11827-11832 (2013-07-03)
Centromeres are chromosomal loci required for accurate segregation of sister chromatids during mitosis. The location of the centromere on the chromosome is not dependent on DNA sequence, but rather it is epigenetically specified by the histone H3 variant centromere protein
Anderly C Chueh et al.
Human molecular genetics, 14(1), 85-93 (2004-11-13)
Human neocentromeres are fully functional centromeres that arise epigenetically from non-centromeric precursor sequences that are devoid of alpha-satellite DNA. Using chromatin immunoprecipitation (ChIP) and BAC-array analysis, we have previously described a 330 kb binding domain for CENP-A (a histone H3

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