T9026

Sigma-Aldrich

Monoclonal Anti-α-Tubulin antibody produced in mouse

enhanced validation

clone DM1A, ascites fluid

Synonym(s):
Monoclonal Anti-α-Tubulin
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DM1A, monoclonal

mol wt

antigen ~50 kDa

contains

15 mM sodium azide

species reactivity

bovine, rat, yeast, human, mouse, chicken, fungi, amphibian

enhanced validation

independent ( antibodies)
Learn more about Antibody Enhanced Validation

application(s)

indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: 1:500 using human or chicken fibroblasts

isotype

IgG1

conjugate

unconjugated

Featured Industry

Research Pathology

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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General description

α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.

Specificity

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

Immunogen

microtubules from chicken embryo brain.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in western blot analysis and protein expression analysis.
Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in the detection of α-Tubulin:
  • in human osteosarcoma and in breast cancer cell lines by western blotting
  • in HeLa cells by immunofluorescence microscopy
  • by immunostaining
  • immunohistochemical detection in Xenopus embryos

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α/β-Tubulin is the major building block of microtubules. These intracellular, hollow, cylindrical, filamentous structures are present in virtually all eukaryotic cells. Self-assembly of α/β-tubulin leads to polar microtubular structures built from linearly arranged strings of alternatively α- and β-tubulin pairs pointing in the same direction. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β-Tubulin is a heterodimer which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  4. What is the immunogen for Monoclonal Anti-α-Tubulin antibody, clone DM1A?

    The immunogen for Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A is microtubules from chicken embryo brain.  This antibody recognizes an epitope located at the C-terminal end of the α-tubulin isoform (amino acids 426-430) as noted in the following references:Breitling, F., and Little, M., J. Molec. Biol., 189, 367-370 (1986).Wolff, A., et al., Biol. Cell, 63, 319-326 (1988).Serrano, L., et al., Anal. Biochem., 159, 253-259 (1986).

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TRAIL induces apoptosis in triple-negative breast cancer cells with a mesenchymal phenotype.
Rahman M, et al.
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p38 Mitogen-activated protein kinase-and HuR-dependent stabilization of p21Cip1 mRNA mediates the G1/S checkpoint.
Lafarga V, et al.
Molecular and Cellular Biology, 29(16), 4341-4351 (2009)
CHIP overexpression reduces mutant androgen receptor protein and ameliorates phenotypes of the spinal and bulbar muscular atrophy transgenic mouse model.
Adachi H
The Journal of Neuroscience, 27(19), 5115-5126 (2007)
Unconventional functions of microtubule motors.
Muresan V and Muresan Z
Archives of Biochemistry and Biophysics, 520(1), 17-29 (2012)
Articles
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
Read More
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
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