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T7451

Sigma-Aldrich

Anti-Acetylated Tubulin antibody, Mouse monoclonal

clone 6-11B-1, purified from hybridoma cell culture

Synonym(s):
Acetylated Tubulin Antibody, Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6-11B-1, monoclonal

form

buffered aqueous solution

species reactivity

plant, hamster, rat, mouse, human, pig, monkey, frog, invertebrates, bovine, protista, chicken

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

dot blot: suitable
electron microscopy: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: 0.03-0.06 μg/mL using total rat brain extract

isotype

IgG2b

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Related Categories

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35.[15] The gene codes for a member of the α -tubulin family containing 448 amino acids.[62][15] TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.[60]
Monoclonal Anti-Acetylated Tubulin (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin;[95] both subunits have a molecular weight of 50,000 and share considerable homology.

Specificity

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates.[14] The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin,[12] within four residues of Lys40 when this amino acid is acetylated.[13] A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corre­sponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast.[13] Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2.[15]

Immunogen

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

Application

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has also been used in quantitative dot blot, immunofluorescence, Western blot,[93] immunocytochemistry,[94] enzyme linked immunosorbent assay (ELISA), solid phase radioimmunoassay (RIA) and electron microscopy.
Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in western blot and immunohistochemistry.[53][56]

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.[63][62][15]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

SDS

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