C7661

Collagen from rat tail

Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture

• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• NACRES: NA.75

Properties

• biological source: rat tail
• Quality Level: 200
• sterility: non-sterile
• product line: BioReagent
• form: powder
• packaging: glass bottle of 5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• solubility: 0.1 M acetic acid: 1 mg/mL (Allow to stir at room temperature 1-3 hours until dissolved.)
• UniProt accession no.: P02454
• shipped in: dry ice
• storage temp.: 2-8°C
• Gene Information: rat ... Col1a1(29393)

Description

General description

Col1a1 (collagen, type I, α1) or collagen is a major structural human protein, which assembles in the form of fibrils. It is a very long, thin and the most abundant protein found in the human body. It is a supercoiled right-helix of three left-handed polypeptide chains. These chains are composed of ~1040 amino acids, which are essentially repeats of three amino acids -(Gly-X-Y)n. Gly is glycine and X and Y can be any amino acids, but in humans are usually proline and hydroxyproline, respectively.

Application

Collagen from rat tail is used for the following applications:

• Immunohistochemistry
• Cellular activity assays
• Used in generation of dorsal root ganglion (DRG) explant cultures
• Used as one of the components during the preparation of the functionalized surface (in NMR setup)
• Used in cell culture (the glass coverslips were coated with nanowires at high concentrations mixed with collagen)
• Used for biofunctionalization of the microchannels

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm². It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Packaging

5 mg in glass bottle

10, 25, 50, 100 mg in poly bottle

Biochem/physiol Actions

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Preparation Note

This product was prepared by a modification of the extraction method of Bornstein, M.B., Lab. Invest., 7, 134 (1958). It is soluble in 0.1 M acetic acid at 1 mg/mL and should be stirred at room temperature for 1-3 hours until dissolved.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves