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C3144

Sigma-Aldrich

Coenzyme A sodium salt hydrate

cofactor for acyl transfer

Synonym(s):
CoA Na2
Empirical Formula (Hill Notation):
C21H36N7O16P3S · xNa+ · yH2O
CAS Number:
Molecular Weight:
767.53 (anhydrous free acid basis)
EC Number:
PubChem Substance ID:
NACRES:
NA.51

description

cofactor for acyl transfer

Quality Level

assay

≥85% (spectrophotometric assay)

form

powder

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

[Na+].CC(C)(COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP(O)([O-])=O)n2cnc3c(N)ncnc23)C(O)C(=O)NCCC(=O)NCCS

InChI key

SYTRWOCXZXQBPW-CLVRNSBASA-M

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General description

Coenzyme A (CoA) is an essential cofactor in living systems and is synthesized from pantothenic acid (vitamin B5), The CoA levels in mitochondria and peroxisomes correspond to 2-5 mM and 0.7 mM, respectively. Cytosolic CoA is in the range of 0.05 mM to 0.14 mM

Application

Coenzyme A is suitable for use in:
  • gylcerolipid biosynthesis in porcine adipose tissue
  • an assay to measure the level of Alpha-methylacyl-CoA racemase (AMACR) in human blood samples using a nanoparticle electrochemical biosensor
  • chloramphenicol acetyltransferase (CAT) assay
  • the synthesis of palmitoyl-CoA, which is required for palmitoylation and activation of proteins for regulated membrane fusion

Packaging

10 mg in glass bottle
25, 100, 500 mg in poly bottle
1 g in poly bottle

Biochem/physiol Actions

Coenzyme A (CoA, CoASH, HSCoA) is a coenzyme that facilitates enzymatic acyl-group transfer reactions and supports the synthesis and oxidation of fatty acids. CoA is involved in the mechanisms of a wide variety of enzymes. In the presence of CoASH, organic carboxylic acids form acyl-CoA thioesters, which facilitates enzyme recognition. The acyl-CoA formed from xenobiotic carboxylic acids can add to the compound′s toxicity, which can lead to cellular metabolic dysfunction. It is involved in the oxidation of pyruvate in the Kreb′s cycle. CoA is needed for metabolic events. The bacterial CoA pathway is targeted for antimicrobial development. It mediates acyl group transfer and carbonyl activation. The CoA and its thioester levels are crucial for cellular homeostasis. CoA is also involved in regulating platelet aggregation and vasoconstriction. It acts as an essential cofactor in enzymatic acetyl transfer reactions.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. C3144.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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