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A3352

Sigma-Aldrich

Acyl-coenzyme A Synthetase from Pseudomonas sp.

≥2 units/mg protein

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Synonym(s):
Acid: CoA ligase (AMP-forming)
CAS Number:
9013-18-7
Enzyme Commission number:
6.2.1.3 (BRENDA, IUBMB)
EC Number:
232-747-5
MDL number:
MFCD00130343

form

powder

Quality Level

200

specific activity

≥2 units/mg protein

shipped in

dry ice

storage temp.

−20°C

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Sigma-Aldrich

Sigma-Aldrich

A3352

Acyl-coenzyme A Synthetase from Pseudomonas sp.

L-Alanine Dehydrogenase from Bacillus subtilis ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

Sigma-Aldrich

A7189

L-Alanine Dehydrogenase from Bacillus subtilis

Sigma-Aldrich

Sigma-Aldrich

L5135

L-Leucine Dehydrogenase from Bacillus cereus

L-Glutamine Synthetase from Escherichia coli lyophilized powder, 400-2,000 units/mg protein

Sigma-Aldrich

G1270

L-Glutamine Synthetase from Escherichia coli

form

powder

form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized powder

specific activity

≥2 units/mg protein

specific activity

≥20 units/mg protein (Lowry)

specific activity

≥60 units/mg protein

specific activity

400-2,000 units/mg protein

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.

Application

Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:
  • as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)
  • in in vitro fatty acylation assays
  • in the synthesis of (14C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (3H)Palmitate-CoA

Acyl-coenzyme A Synthetase may be used to study fatty acid metabolism and lipid metabolism. It has been used to study its interaction with fatty acid transport proteins, which has been found to be involved in the efficient cellular uptake of long-chain fatty acids in adipocytes .

Biochem/physiol Actions

Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells .
Acyl-coenzyme A (CoA) synthetase (ACS) enzyme catalyzes a two-step thioesterification reaction involving the conversion of free fatty acids (FAs) to CoA esters. The substrate for ACS varies from two carbon to 26 carbon FAs. It is involved in the activation of FAs for lipid metabolism and enables FA to participate in various cellular metabolic pathways.

Unit Definition

One unit will form 1.0 μmole of AMP and oleoyl coenzyme A from ATP and oleate at pH 8.1 at 25 °C in the presence of Coenzyme A.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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