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A1765

Sigma-Aldrich

S-Acetyl-coenzyme A synthetase from baker's yeast (S. cerevisiae)

lyophilized powder, ≥3 units/mg protein

All Photos(1)
Synonym(s):
Acetate CoA ligase (AMP forming), Acetate thiokinase
CAS Number:
9012-31-1
Enzyme Commission number:
6.2.1.1 (BRENDA, IUBMB)
EC Number:
232-729-7
MDL number:
MFCD00081267
NACRES:
NA.26

form

lyophilized powder

Quality Level

200

specific activity

≥3 units/mg protein

composition

Protein, 10-30% biuret

storage temp.

−20°C

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567422PH001619PH003326
Sigma-Aldrich

Sigma-Aldrich

A1765

S-Acetyl-coenzyme A synthetase from baker's yeast (S. cerevisiae)

Sodium Acetate, 3M, pH 5.2, Molecular Biology Grade Sterile solution. Prepared with DEPC for RNA applications. Also suitable for DNA applications.

Millipore

567422

Sodium Acetate, 3M, pH 5.2, Molecular Biology Grade

N-[4-[2-((2S)-5-[[2-tert-Butyl-4-(hydroxymethyl)-5-methylphenyl]sulfanyl]-4-hydroxy-2-isopropyl-6-oxo-3,6-dihydro-2H-pyran-2-yl)ethyl]phenyl]acetamide AldrichCPR

Sigma-Aldrich

PH001619

N-[4-[2-((2S)-5-[[2-tert-Butyl-4-(hydroxymethyl)-5-methylphenyl]sulfanyl]-4-hydroxy-2-isopropyl-6-oxo-3,6-dihydro-2H-pyran-2-yl)ethyl]phenyl]acetamide

1,3,4,6-Tetra-O-acetyl-2-deoxy-2-{[(e)-(4-methoxyphenyl)methylidene]amino}-b-d-glucopyranose AldrichCPR

Sigma-Aldrich

PH003326

1,3,4,6-Tetra-O-acetyl-2-deoxy-2-{[(e)-(4-methoxyphenyl)methylidene]amino}-b-d-glucopyranose

form

lyophilized powder

form

liquid

form

-

form

-

specific activity

≥3 units/mg protein

specific activity

-

specific activity

-

specific activity

-

storage temp.

−20°C

storage temp.

10-30°C

storage temp.

-

storage temp.

-

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

-

composition

Protein, 10-30% biuret

composition

-

composition

-

composition

-

Application

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae) has been used in the synthesis of adenosine 5′-tetraphosphate and adenosine 5′-pentaphosphate.
S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO2 fixation. It may also be used in gene expression studies.

Biochem/physiol Actions

Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol .

Packaging

Package size based on protein content.

Unit Definition

One unit will form 1.0 μmole of S-acetyl coenzyme A from acetate, ATP, and coenzyme A per min at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing stabilizers as potassium phosphate, sucrose, and reduced glutathione

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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