A1470

Bovine Serum Albumin

low endotoxin, lyophilized powder, Cohn Analog^™, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• NACRES: NA.27

Properties

• biological source: bovine
• Quality Level: 200
• product line: BioReagent
• Assay: ≥98% (agarose gel electrophoresis)
• form: lyophilized powder
• mol wt: ~66 kDa
• purified by: heat shock fractionation
• origin: USA origin
• technique(s): cell culture | mammalian: suitable
• impurities: ≤20 ppm Pb; ≤3 EU/mg endotoxin
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt accession no.: P02769
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

Application

Recommended as a growth-enhancing supplement for cell culture.
Select the right albumin for your application with the Albumin Selection Guide

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

• Tested for use in cell culture
• Low endotoxin

Preparation Note

Manufactured by a novel process that maintains the lipid and growth factor characteristics of cold-ethanol precipitated albumins.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Legal Information

Cohn Analog is a trademark of Proliant, Inc.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves